Glycosylation Patterns Attacked by CT Discussion Paper

CHEM 5620 Protein Chemistry

Assignment 06

 

This assignment is about these things: Cholera (caused by Vibrio cholera)

Diarrhea (caused by enterotoxigenic Escherichia coli)

Whooping cough (caused by Bordetella pertussis)

Dysentery (caused by Shigella dysenteriae)

Typhoid fever (caused by Salmonella typhi)

Diphtheria (caused by Corynebacterium diphtheriae)

 

 

 

  1. Use the papers made available on Canvas as a starting point to comment on how the cholera toxin (CT) is connected to each of the following aspects of protein chemistry. What I’m looking for here is a demonstration that you can read about new proteins and discuss how each of these things might apply. A good way to approach this is to first define each of the items, then describe how it relates to cholera toxin.

 

    1. KDEL sequence

 

    1. PDI (specifically in the CT lifecycle)

 

    1. Glycosylation patterns on the surface of cells attacked by CT

 

    1. Secretion of the toxin from V. cholerae (how does it get out)

 

    1. Endocytosis into target cells (how does it get in)

 

    1. The Sec61 channel and ERAD

 

    1. 2° and 3° similarity between CT and other toxin protein

 

 

 

 

  1. Use both text and Richardson diagrams to do the following:

 

    1. Describe the domain arrangement of the typhoid holotoxin.

 

    1. Describe the molecular basis for why typhoid infects humans but not chimpanzees.

 

 

 

  1. Explain what CRM197 is and why is it useful. The more detailed your answer about what it is (sequence, structure, function, etc.), the easier it will be to explain why it is useful. Searching in Pubmed is a great way to get started on this question,

 

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