Metabolic Pathways Project
The goal of this project is to apply the concepts and ideas from the lectures of BCH 361 to other related metabolic pathways. This project will be submitted in parts to Turnitin via D2L.
- Select a topic from this list. Sign up for the topic using the project group sign up on D2L. Each is limited to 50 students. Sign up early for the best chance of getting your first choice.
- Topic 1: Entner-Douderoff Pathway
- Topic 2: L-Ascorbate Biosynthesis
- Topic 3: D-Tagatose Degradation
- Topic 4: L-Rhamnose Degradation
- Topic 5: L-Arabinose Degradation
- Topic 7: meso-Tartaric Acid Metabolism
- Topic 8: Glyoxylate Cycle
- Research your topic. You don’t need to do all of this at once. Plan your work around the submission deadlines and pace yourself so you don’t try to do it all at the end.
- What is the pathway? (Sometimes there are multiple pathways; choose the most appropriate one(s)). You need to show full structures for each of the substrates and identify the enzyme that catalyzes each reaction. Is this pathway similar to any other pathway we have discussed in the course?
- What organisms use the pathway?
- What is the purpose or importance of the pathway?
- How do the enzymes work (what are the mechanisms)? How do these compare to mechanisms of other enzymes discussed in the lectures?
- How is the pathway regulated? (This may not be known; make inferences and back up your position)
- Does the pathway feed into glycolysis, or the TCA cycle, or some other pathway?
- How much energy is generated (or used) in the pathway in terms of molecules such as ATP, NAD(P), and FAD?
- Prepare and submit an annotated bibliography.
- Identify at least three journal articles that can serve as sources for your project. For three of these articles, outline in no more than three sentences per article the information it will provide towards your project. This does not need to be your entire reference list!
- Page limit = ½ page.
- SUBMISSION DEADLINE: 2 October 2020 4. Prepare and submit your Introduction.
- This report will include parts a-c in the above Research section.
- Include the pathway (each has at least two steps) showing the structures of the metabolites and naming each enzyme. Draw this out yourself; do not copy and paste from the literature or websites, but do cite your source(s).
- Include your reference list of works cited in the outline and introduction.
- Page limit = 2 pages
- SUBMISSION DEADLINE: 30 October 2020
[Type here] 1 BCH 361 Advanced Biochemistry I Fall 2020
5. Prepare and submit your final report.
- This report will focus on parts d-g in the above Research section. Include your Introduction (parts a-c).
- Draw the mechanism for at least one of the reactions that is not a simple phosphorylation/dephosphorylation. If possible, indicate the active site residues that are involved in the mechanism.
- You may wish to divide your report into sections as is normally done in writing review articles.
- Include your reference list with all the works cited.
- Page limit = 6 pages including Introduction
- SUBMISSION DEADLINE: 4 December 2020
- Submission Guidelines.
Prepare your work as a PDF or DOC file for submission to Turnitin on D2L. Work exceeding the page limits will not be graded. Please be aware of the different submission deadlines and plan your work accordingly. Late work will be penalized. Early submissions are encouraged.
Formatting: Your work will be double-spaced and have 1 inch (2.5 cm) margins, and use a standard 11 or 12 point business font. The reference section will be single spaced for each reference, and double spaced between references. If your work has more than one page, number the pages. Make sure your name is on the upper right corner of the first page of your work.
Drawing structures and pathways: You may draw the structures by hand and photograph or scan them, or use software such as ChemDraw. Pyranose forms of sugars should be in a chair, boat, or twist-boat conformation. Do not show Haworth projections.
- Originality and Academic Integrity.
You are encouraged to be original in your presentation with regards to the perspective you take. However, you must acknowledge the work of others (your sources) using a standard format. Be consistent. A good format for your references and citations would be to use the ACS Biochemistry format (like in the lab), but to add in the title of the journal article.
Do not quote your sources unless you want to comment on their wording. Instead, paraphrase. Student Learning Support offers a number of workshops, some of which deal with paraphrasing and citations.
Failure to acknowledge your sources could result in an investigation for academic misconduct as outlined in Policy 60.
If you are unsure what to do, or realize that you made an error, contact Dr. Johnson ASAP.
Guidance on how each of these will be graded will be provided closer to the submission deadlines.
[Type here] 2